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foxp3 fixation/permeabilization working solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher foxp3 fixation/permeabilization working solution
    Foxp3 Fixation/Permeabilization Working Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeabilization+working+solution/foxp3+transcription+factor+staining+buffer+set/pm40664449-116-7-19
    Average 90 stars, based on 1 article reviews
    foxp3 fixation/permeabilization working solution - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Liangxue Xiaoban decoction and its disassembled prescriptions ameliorate psoriasis-like skin lesions induced by imiquimod in mice via T cell regulation
    Article Snippet: The CD8 (100731), CD25 (102036), IL-17A (506915), IFN-g (505836), and IL-4 (504131) antibodies were purchased from BioLegend (San Diego, CA). .. The FOXP3 antibody (25-5773-82), cDNA synthesis kit (K1622), permeabilization working solution, and permeabilization buffer (00-5523-00) were purchased from Thermo Fisher Scientific (Waltham, MA). ..

    Incubation:

    Article Title: Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells
    Article Snippet: Briefly, the blood cells were incubated with anti-mouse CD4-APC (cat. no. 17-0041-81; eBioscience; Thermo Fisher Scientific, Inc.) and anti-mouse CD25-PE-Cy7 antibodies (cat. no. 25-0251-81; eBioscience; Thermo Fisher Scientific, Inc.) for 30 min at 4°C, and then lysed with ACK lysis buffer (Thermo Fisher Scientific, Inc.) to remove red blood cells. .. The remaining white blood cells (WBCs) were treated with fresh fixation/permeabilization working solution (Thermo Fisher Scientific, Inc.) for 30 min at room temperature, then incubated with anti-mouse Foxp3-PE antibody (cat. no. 12-5773-82; eBioscience; Thermo Fisher Scientific, Inc.) in fresh permeabilization working solution (Thermo Fisher Scientific, Inc.) for another 30 min at 4°C. .. For cytokine staining, blood was diluted two-fold in RPMI 1640, stimulated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (Merck KGaA) for 4 h, and then treated with 1 μg/ml monensin (Merck KGaA) for another 2 h. Cytokine staining was subsequently performed following the manufacturers' instructions for the two-step protocol for intracellular (cytoplasmic) proteins.

    Article Title: Human labour is associated with altered regulatory T cell function and maternal immune activation
    Article Snippet: FoxP3 staining Following surface staining and prior to fixation, PBMC were washed and resuspended in 1 ml of freshly prepared FoxP3 fixation/permeabilization working solution, consisting of FoxP3 fixation/permeabilization concentrate (eBioscience, Hatfield, UK) diluted with FoxP3 fixation/permeabilization diluent (eBioscience) at a concentration of 1 : 3, and incubated in the dark for 30 min at +4°C. .. The mixture was washed in permeabilization working solution (eBioscience), resuspended in the same solution and 5 μl phycoerythrin (PE)‐labelled anti‐FoxP3 was added and incubated in the dark at room temperature for 30 min. After two wash steps the cells were resuspended in 200 μl of diluted stabilizing fixative and stored at 4°C until acquisition. ..

    Article Title: Cancer immunotherapy via synergistic co-activation of myeloid receptors CD40 and Dectin-1
    Article Snippet: Thawed cells were counted, washed and 2×10 6 cells or fewer per sample were barcoded by incubation with palladium metal tags as per the manufacturer’s instructions (Fluidigm). .. Pooled cells were then incubated with permeabilization working solution (EBioscience) with 2% mouse serum and mouse anti-CD16/32 and stained with a lymphoid or myeloid focused antibody master mix ( Table S4 and S5 ) for 30 minutes at room temperature. .. Cells were washed and fixed in 2.4% formaldehyde with iridium nucleic acid intercalator (125 nM, Fluidigm) overnight at 4°C.

    Staining:

    Article Title: Cancer immunotherapy via synergistic co-activation of myeloid receptors CD40 and Dectin-1
    Article Snippet: Thawed cells were counted, washed and 2×10 6 cells or fewer per sample were barcoded by incubation with palladium metal tags as per the manufacturer’s instructions (Fluidigm). .. Pooled cells were then incubated with permeabilization working solution (EBioscience) with 2% mouse serum and mouse anti-CD16/32 and stained with a lymphoid or myeloid focused antibody master mix ( Table S4 and S5 ) for 30 minutes at room temperature. .. Cells were washed and fixed in 2.4% formaldehyde with iridium nucleic acid intercalator (125 nM, Fluidigm) overnight at 4°C.



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